The fine powders of young tubers of Hydnophytum formicarum was extracted by Soxhlet methanol extraction for 18 hours in three days continuously. The filtrate then was evaporated by rotary evaporator until it became concentrated solution. The fractionation and purification of the extract by vacuum liquid chromatography and radial chromatography has led to the discovery of four compounds namely sinapinic acid, β-sitosterol acetate, β-sitosterol and stigmasterol. Structures of the compounds were established by interpreting mass spectral data, 1H and 13C-APT NMR, Infrared and by comparison with literature data.