A simple preparation of functional nanoscale graphene oxide (GO) via synthetic route was done using modified Hummer’s Method whilst cellulose nanofiber from oil palm empty fruit bunch fiber was prepared using acid hydrolysis method. An active polylactide based nanocomposite film was prepared by incorporation of cellulose nanofiber (CNF), graphene oxide (GO) and essential oil (EO). In determining of factor influences the mechanical properties (tensile strength, elongation percentage and Young’s modulus), the response surface methodology (RSM) Box Behnken Design (BBD) were used. The factors considered were the ratio (wt.%) of GO and CNF as an additive and 5 wt.% of EO was set as minimum. The mechanical properties that interpreted as tensile strength, percent elongation and Young’s modulus were the response variables investigated. The ratio of EO wt.% (C) is found to be the most significant factor that influences the tensile strength of the nanocomposite. In the case of elongation percentage (%E) the percentage of cellulose nanofiber CNF (A) gave the most significant effect, where in Young’s modulus, EO wt.% (C) is the most significant effect, followed by wt.% of GO (B). Validation of optimization by carrying out the confirmation run high degree of prognostic ability of response surface methodology. The results showed that the optimized formulation provided a mechanical strength (tensile strength, percentage elongation and Young’s Modulus) pattern that is similar to the predicted curve, which indicated that the optimal formulation could be obtained using RSM.