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Molecular cloning and in silico characterization of fructansucrase gene from Weissella confusa MBFCNC-2(1) isolated from local beverage
Malik, Amarila1.
Fructan-type exopolysaccharides (EPS) (inulin and levan) and their oligosaccharides (fructooligosaccharides/FOS) are of great interest to the food and pharmaceutical industries. EPS producing-lactic acid bacteria (LAB) have been reported to produce -fructans (both inulin and levan) as well as α-glucans through the action of sucrase enzymes fructansucrase (FS) and
glucansucrase (GS). The Weissella confusa strain MBFCNC-2(1), isolated from a sample of a local beverage, and was shown to possess the open reading frame (ORF) of the FS coding gene (ftf) with high similarity to a putative inulosucrase of L. reuteri, which was designated ftfCNC-2(1). In total, 2,137 bp of full length ftfCNC-2(1) DNA was successfully cloned and sequenced, and a putative FTF protein of 900 amino acids was analyzed. A core region was identified as an active domain belonging to glycoside hydrolase 68. The N-terminal region contained a signal sequence followed by a sequence similar to the N-terminus of gtfKg15 (putatively identified as glucansucrase); a putative substrate binding domain was interestingly shown to be Serine Threonin
rich. The C-terminus region contained an LPXTG motif, a cell wall anchoring domain, which was also Threonin rich. For further study, the ftfCNC-2(1) gene was successfully subcloned into a shuttle vector for future expression in either E. coli or B. subtilis.
Affiliation:
- Universitas Indonesia, Indonesia
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Indexation |
Indexed by |
MyJurnal (2019) |
H-Index
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0 |
Immediacy Index
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0.000 |
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0 |
Indexed by |
Scopus (SCImago Journal Rankings 2016) |
Impact Factor
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Rank |
Q4 (Biotechnology) Q4 (Molecular Biology) |
Additional Information |
0.137 (SJR) |
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