The unique and isolated nature of oxbow lakes from its parent river is reflected by its water quality. This study was carried out to determine the water quality of oxbow lake located along the Sg. Sugut, Beluran, Sg. Padas, Beaufort and Sg. Kinabatangan, Sandakan, Sabah with different human activities. Physical and chemical parameters studied on site were dissolved oxygen (DO), temperature, pH value, conductivity and secchi disk depth (SDD) while those analysed in the laboratory were total suspended solid (TSS), total phosphorus (TP) and chlorophyll-a. From the results indicate human activities near oxbow lake cause increment of nutrient. It will lead ecotone by eutrophication that start succession of water bodies to terrestrial land. Number of oxbow lakes and its uniqueness will diminish and its function as organisms sanctuary will be greatly affected.