Titanium dioxide nanoparticle dispersions interaction with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles is reported in this paper. DPPC- TiO2 interactions were investigated using dynamic light scattering (DLS) and transmission electron microscopy (TEM) by focusing on the effect of the interactions on the surface charge, size and localisation of TiO2 in DPPC- TiO2 vesicles. It was observed that surface charge of DPPC- TiO2 vesicles were increased from -26.0 to 12.1 mV as the TiO2 nanoparticle concentrations increased from 0.01 to 100 mg/ml. The binding of positively charged TiO2 nanoparticle to the zwitterionic DPPC vesicles caused the surface charge to become more positive. The size distribution, localisation and positioning of TiO2 nanoparticles in DPPC vesicles were confirmed through TEM analysis.